Current events

Date: Feb 24th

Time: 09:30 am

Place : 2nd Floor Conference Room, Gonda building.

Title : "Inducible and Selective Erasure of Memories in the Mouse Brain via Chemical-Genetic Manipulation"

Speaker: Adam Frank.

Summary:

I will present Joe Tsien's 2008 Neuron paper about a chemical-genetic approach to study CaMKIIa function ("Inducible and Selective Erasure of Memories in the Mouse Brain via Chemical-Genetic Manipulation"). This paper is a continuation of work beginning in 2003, when his lab generated a CaMKIIa overexpressing mouse with a targeted mutation in CaMKIIa that makes it highly susceptible to inhibition by a modified kinase inhibitor (Wang, 2003, PNAS). This story of overexpression and inhibition of wildtype CaMKIIa activity is interesting and provocative and raises as many questions as it answers. I am particularly interested in these results as I have generated a BAC transgenic mouse that overexpresses the same CaMKIIa mutation as that generated in the Tsien lab. I am hopeful we can have a lively discussion about his results and the important questions they raise. Relevant Reading Material:

Neuron. 2008 Oct 23;60(2):353-66. Inducible and selective erasure of memories in the mouse brain via chemical-genetic manipulation. Cao X, Wang H, Mei B, An S, Yin L, Wang LP, Tsien JZ.

Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4287-92. Epub 2003 Mar 19. Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain. Wang H, Shimizu E, Tang YP, Cho M, Kyin M, Zuo W, Robinson DA, Alaimo PJ, Zhang C, Morimoto H, Zhuo M, Feng R, Shokat KM, Tsien JZ.